Authors
RM De Lederkremer, MJM Alves, GC Fonseca, W Colli
Publication date
1976/8/24
Journal
Biochimica et Biophysica Acta (BBA)-General Subjects
Volume
444
Issue
1
Pages
85-96
Publisher
Elsevier
Description
A lipopeptidophosphoglycan was extracted from epimastigote forms of Trypanosomacruzi by phenol (44%) treatment of sonicated cells. The substance was purified from other glycoproteins and nucleic acid as follows: ethanol frationation, Bio-Gel P-150 column chromatography in the presence of 0.1% sodium dodecyl sulfate, extraction with chloroform/methanol/water (10 : 10 : 3) and precipitation of the pure compound by methanol. The substance migrated as a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis stained with periodic acid-Schiff and Coomassie blue. In the absence of sodium dodecyl sulfate very little or no migration was observed in 5% and 10% of the gels respectively, suggesting the formation of aggregates. In such gels a Sudan Black positive reaction coincident with the periodic acid-Schiff positive band was obtained. Neutral sugars (60%, by phenol-sulfuric acid assay …
Total citations
Scholar articles
RM De Lederkremer, MJM Alves, GC Fonseca, W Colli - Biochimica et Biophysica Acta (BBA)-General Subjects, 1976
RM de Lederkremer, CT Tanaka, MJM ALVES, W Colli - European Journal of Biochemistry, 1977
RM Lederkremer, OL Casal, MJM Alves, W Colli - 1981