Authors
Chantal BF Vogels, Anderson F Brito, Anne L Wyllie, Joseph R Fauver, Isabel M Ott, Chaney C Kalinich, Mary E Petrone, Arnau Casanovas-Massana, M Catherine Muenker, Adam J Moore, Jonathan Klein, Peiwen Lu, Alice Lu-Culligan, Xiaodong Jiang, Daniel J Kim, Eriko Kudo, Tianyang Mao, Miyu Moriyama, Ji Eun Oh, Annsea Park, Julio Silva, Eric Song, Takehiro Takahashi, Manabu Taura, Maria Tokuyama, Arvind Venkataraman, Orr-El Weizman, Patrick Wong, Yexin Yang, Nagarjuna R Cheemarla, Elizabeth B White, Sarah Lapidus, Rebecca Earnest, Bertie Geng, Pavithra Vijayakumar, Camila Odio, John Fournier, Santos Bermejo, Shelli Farhadian, Charles S Dela Cruz, Akiko Iwasaki, Albert I Ko, Marie L Landry, Ellen F Foxman, Nathan D Grubaugh
Publication date
2020/10
Journal
Nature microbiology
Volume
5
Issue
10
Pages
1299-1305
Publisher
Nature Publishing Group
Description
The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription–PCR (RT–qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from different tests are comparable. Our goal was to make independent evaluations of primer–probe sets used in four common SARS-CoV-2 diagnostic assays. From our comparisons of RT–qPCR analytical efficiency and sensitivity, we show that all primer–probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction. The exception for this is the RdRp-SARSr (Charité) confirmatory primer–probe set which has low sensitivity, probably due to a mismatch to circulating SARS-CoV-2 in the reverse primer …
Scholar articles
CBF Vogels, AF Brito, AL Wyllie, JR Fauver, IM Ott… - Nature microbiology, 2020
CBF Vogels, AF Brito, AL Wyllie, JR Fauver, IM Ott… - MedRxiv, 2020