Authors
Wouter AA de Steenhuijsen Piters, Elisabeth GW Huijskens, Anne L Wyllie, Giske Biesbroek, Menno R Van Den Bergh, Reinier H Veenhoven, Xinhui Wang, Krzysztof Trzciński, Marc J Bonten, John WA Rossen, Elisabeth AM Sanders, Debby Bogaert
Publication date
2016/1
Journal
The ISME journal
Volume
10
Issue
1
Pages
97-108
Publisher
Oxford University Press
Description
Bacterial pneumonia is a major cause of morbidity and mortality in elderly. We hypothesize that dysbiosis between regular residents of the upper respiratory tract (URT) microbiome, that is balance between commensals and potential pathogens, is involved in pathogen overgrowth and consequently disease. We compared oropharyngeal microbiota of elderly pneumonia patients (n=100) with healthy elderly (n=91) by 16S-rRNA-based sequencing and verified our findings in young adult pneumonia patients (n=27) and young healthy adults (n=187). Microbiota profiles differed significantly between elderly pneumonia patients and healthy elderly (PERMANOVA, P<0.0005). Highly similar differences were observed between microbiota profiles of young adult pneumonia patients and their healthy controls. Clustering resulted in 11 (sub)clusters including 95% (386/405) of samples. We observed three microbiota …
Scholar articles
WAA de Steenhuijsen Piters, EGW Huijskens… - The ISME journal, 2016