Authors
Sabine MPJ Prevaes, Karin M De Winter-De Groot, Hettie M Janssens, Wouter AA de Steenhuijsen Piters, Gerdien A Tramper-Stranders, Anne L Wyllie, Raiza Hasrat, Harm A Tiddens, Mireille Van Westreenen, Cornelis K Van Der Ent, Elisabeth AM Sanders, Debby Bogaert
Publication date
2016/3/1
Journal
American journal of respiratory and critical care medicine
Volume
193
Issue
5
Pages
504-515
Publisher
American Thoracic Society
Description
Rationale: Cystic fibrosis (CF) is characterized by early structural lung disease caused by pulmonary infections. The nasopharynx of infants is a major ecological reservoir of potential respiratory pathogens.
Objectives: To investigate the development of nasopharyngeal microbiota profiles in infants with CF compared with those of healthy control subjects during the first 6 months of life.
Methods: We conducted a prospective cohort study, from the time of diagnosis onward, in which we collected questionnaires and 324 nasopharynx samples from 20 infants with CF and 45 age-matched healthy control subjects. Microbiota profiles were characterized by 16S ribosomal RNA–based sequencing.
Measurements and Main Results: We observed significant differences in microbial community composition (P < 0.0002 by permutational multivariate analysis of variance) and development between groups. In infants with CF, early …
Scholar articles
SMPJ Prevaes, KM De Winter-De Groot, HM Janssens… - American journal of respiratory and critical care …, 2016