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In order to improve the human resource quality in university, it is required high lecturer quality and to get good lecturer quality, it is required job satisfaction. The level of job satisfaction is influenced by stress level and the experienced multiple role conflict. This research is conducted to know the effect of the multiple role conflict to the stress and job satisfaction level to the university female lecturer. The research is conducted to two private universities by giving 143 questionnaires to full-time and part-time lecturers. The data is processed by using Partial Least Square with analysis of Structural Equation Model (PLS-SEM). This research finds a significant positive relation between the multiple role conflicts on stress level, there is also negative and significant relation between stress levels on job satisfaction and there is negative and insignificant relation between the multiple role conflicts on job satisfaction. The multiple role conflict has no direct relation with the job satisfaction; these two relations are mediating by stress.