Authors
Elaine R Nimmo, James G Prendergast, Marian C Aldhous, Nicholas A Kennedy, Paul Henderson, Hazel E Drummond, Bernard H Ramsahoye, David C Wilson, Colin A Semple, Jack Satsangi
Publication date
2011/10/21
Journal
Inflammatory bowel diseases
Volume
18
Issue
5
Pages
889-899
Publisher
Oxford University Press
Description
Background
Germline variation in the 71 Crohn's disease (CD) loci implicated by genome-wide association studies (GWAS) only accounts for approximately 25% of estimated heritability. The contribution of epigenetic alterations to disease pathogenesis is emerging as a research priority.
Materials and Methods
The methylation status of 27,578 CpG sites across the genome was analyzed using the Illumina Human Methylation27 assay in DNA extracted from whole blood samples from 40 adult females (21 ileal CD, 19 healthy controls) and 16 girls with childhood-onset CD, all nonsmokers. Our primary analysis compared methylation profiles in adult cases and controls.
Results
Our data define a global methylation profile characteristic of ileal CD. In all, 1117 sites were differentially methylated (corrected P < 0.01); 50 showed significantly altered methylation in …
Total citations
Scholar articles
ER Nimmo, JG Prendergast, MC Aldhous, NA Kennedy… - Inflammatory bowel diseases, 2012