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Fire and grazers (such as Bison bison) were historically among the most important agents for maintaining and managing tallgrass prairie, but we know little about their influences on water-quality dynamics in streams. We analyzed 2 y of data on total suspended solids (TSS), total N (TN), and total P (TP) (3 samples per week per stream during flow) in 3 prairie streams with fire and bison grazing treatments at Konza Prairie Biological Station, Kansas (USA), to assess whether fire and bison increase the concentrations of these water-quality variables. We quantified the spatial and temporal locations of bison (∼0.21 animal units/ha) with Global Positioning System collars and documented bison trails, paw patches, wallows, and naturally exposed sediment patches within riparian buffers. Three weeks post-fire, TN and TP decreased (t-test, p < 0.001), but TSS did not change. Bison spent <6% of their time within 10 m of the …