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N Alters, Ras Depalmitoylation, Sharon Eisenberg, Haguy Wolfenson, Ronen Berkovich, Ian A Prior, Marcelo Ehrlich, Joseph Klafter, Michael Urbakh, Yoav I Henis

Quantitative FRAP Analysis Demonstrates that Raft Protein Clustering Alters N-Ras Depalmitoylation, Membrane Interactions and Ac Page 1 details have emerged regarding the mechanism of GPCR-Gabg complex formation during signaling initiation because of the intrinsic instability of such complexes both in vivo and in vitro in the presence of detergent micelles. To overcome this limitation, we examined the utility of a range of membrane mimetics for stabilizing GPCR-Gabg complexes using the model system pair rhodopsin-transducin responsible for visual transduction. This system was chosen for its spectroscopic properties that can be easily followed as a measure of complex stability. Our results support the dependence of GPCR-Gabg complex formation on membrane morphology and nonspecific electrostatic interactions between membrane components and membrane protein binding molecules. 159-Plat …