Authors
Hidetoshi Iida, S Sakaguchi, Y Yagawa, Y Anraku
Publication date
1990/12/5
Journal
Journal of Biological Chemistry
Volume
265
Issue
34
Pages
21216-21222
Publisher
Elsevier
Description
We established an experimental system suitable for study of cell cycle regulation by Ca2+ in the yeast Saccharomyces cerevisiae. Systematic cell cycle analysis using media containing various concentrations of Ca2+, a Ca2(+)-ionophore (A23187), and a Ca2(+)-chelator [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) revealed that simultaneous addition of 10 microM A23187 and 10 mM EGTA to cells growing in a Ca2(+)-deficient medium at 22 degrees C caused rapid decrease in intracellular Ca content and resulted in transient G1 arrest followed by block mostly at G2/M, as revealed by flow cytometry. Recovery from G1 arrest was not due to coordinated initiation of DNA synthesis and bud emergence: unbudded cells with S or G2/M DNA were observed. Examination of terminal phenotype suggested that Ca2+ was required at all the stages of the cell cycle except for the initiation of DNA synthesis. The …
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Scholar articles
H Iida, S Sakaguchi, Y Yagawa, Y Anraku - Journal of Biological Chemistry, 1990