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In this study, we used a DNA fingerprinting technique (PCR-DGGE) to investigate if bacteria present in an activated EM suspension (EM-A) were able to maintain or reach significant relative abundances after addition to slurry manure. We also investigated effects of the addition of EM-A to slurry manure on nitrogen losses from the manure and grass biomass production after application of the manure as organic fertilizer. EM^® and Agri-mest^® were tested in a factorial set-up so that the factors could be distinguished. Effective Organisms (EM) is a mixture of several effective, disease-depressing micro-organisms. Agri-mest contains energized minerals, which will influence biochemical processes occurring in the manure. Different EM-stocks, and EM-Active suspensions prepared by different users showed large variation in bacterial community structure and, thus, low reproducibility. Through the addition of Agri-mest to the …