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Background: Platelet activation and thrombus formation play a key role in non-ST segment elevation acute coronary syndromes (NSTE-ACS). The study of platelet protein expression profile during and after the acute episode would help to understand the pathophysiological changes that take place during the condition and assist in the development of new therapies. Because platelets do not have a nucleus, proteomics is an adequate approach to achieve the above objective, allowing the analysis of thousands of proteins at once.

Methods: We performed a proteomic analysis of circulating platelets from 18 patients with a NSTE-ACS (on admission, at 5 days and 6 months) and 10 controls with stable chronic ischemic cardiopathy. There were no significant differences in age, sex and treatment between groups. Proteins were separated by high-resolution two-dimensional gel electrophoresis and analyzed by mass …