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Purpose: Microparticles (MP) are cell-derived membrane vesicles of less than a micron known to be increased in the blood of patients with acute coronary syndromes. They derive from various cells, most notably platelets. Our objective was to compare the proteome of plasma-derived microparticles from ST-elevation myocardial infarction (STEMI) patients and stable coronary artery disease (SCAD) controls, and to detect MP proteins differentially regulated between both groups.

Methods: Plasma MP were isolated from 10 STEMI patients and 10 SCAD matched controls. We followed a standardized procedure to guarantee the purity of the microparticle population. MPs were characterized by FACS and by electron microscopy. Proteome analysis was based on high-resolution two-dimensional differential in-gel electrophoresis (2D-DIGE) and mass spectrometry. Validations were by 1D- and 2D-western …