Authors
Lu Chen, Myrto Kostadima, Joost HA Martens, Giovanni Canu, Sara P Garcia, Ernest Turro, Kate Downes, Iain C Macaulay, Ewa Bielczyk-Maczynska, Sophia Coe, Samantha Farrow, Pawan Poudel, Frances Burden, Sjoert BG Jansen, William J Astle, Antony Attwood, Tadbir Bariana, Bernard De Bono, Alessandra Breschi, John C Chambers, Bridge Consortium, Fizzah A Choudry, Laura Clarke, Paul Coupland, Martijn van der Ent, Wendy N Erber, Joop H Jansen, Rémi Favier, Matthew E Fenech, Nicola Foad, Kathleen Freson, Chris van Geet, Keith Gomez, Roderic Guigo, Daniel Hampshire, Anne M Kelly, Hindrik HD Kerstens, Jaspal S Kooner, Michael Laffan, Claire Lentaigne, Charlotte Labalette, Tiphaine Martin, Stuart Meacham, Andrew Mumford, Sylvia T Nürnberg, Emilio Palumbo, Bert A van der Reijden, David Richardson, Stephen J Sammut, Greg Slodkowicz, Asif U Tamuri, Louella Vasquez, Katrin Voss, Stephen Watt, Sarah Westbury, Paul Flicek, Remco Loos, Nick Goldman, Paul Bertone, Randy J Read, Sylvia Richardson, Ana Cvejic, Nicole Soranzo, Willem H Ouwehand, Hendrik G Stunnenberg, Mattia Frontini, Augusto Rendon
Publication date
2014/9/26
Journal
Science
Volume
345
Issue
6204
Pages
1251033
Publisher
American Association for the Advancement of Science
Description
Introduction
Blood production in humans culminates in the daily release of around 1011 cells into the circulation, mainly platelets and red blood cells. All blood cells originate from a minute population of hematopoietic stem cells (HSCs) that expands and differentiates into progenitor cells with increasingly restricted lineage choice. Characterizing alternative splicing events involved in hematopoiesis is critical for interpreting the effects of mutations leading to inherited disorders and blood cancers and for the rational design of strategies to advance transplantation and regenerative medicine.
Overview of methodology. RNA-sequencing reads from human blood progenitors [opaque cells in (A)] were mapped to the transcriptome to quantify gene and transcript expression. Reads were also mapped to the genome to identify novel splice junctions and characterize alternative splicing events (B).
Rationale
To address this …
Scholar articles
L Chen, M Kostadima, JHA Martens, G Canu… - Science, 2014