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Small streams with acidic, nutrient-poor waters form a dense hydrological system in Central Amazonia. However, little is known about the fish assemblages that occur in these systems. We investigated the relationships among stream size, substrate, and other physical-chemical factors and fish distributions. Fish assemblages and stream characteristics were sampled in 50-m long sections of streams at 38 sites distributed throughout the 100-km 2 florestal reserve. Forty-nine species were encountered. The mean richness was similar among sites; however, community composition was correlated with physical and chemical characteristics of the streams. The mean number of species per site (X̄= 9) was low in relation to the total number of species recorded from the reserve (49) and in relation to the ichthyofauna that has been recorded from small forest streams near the study area. This contrasts with studies in …