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Risk of metabolic and intestinal inflammatory disorder development increases significantly with age. In contrast, exercise has shown to reduce disease risk and promote longevity. Interestingly, adenylyl cyclase 5 knock out (AC5KO) mice demonstrate an enhanced exercise capacity and improved longevity. We aimed to examine the inflammatory status along the gastrointestinal tract of AC5KO mice compared to Wild type (WT) mice. 21 C57BL WT and AC5KO male mice were randomly assigned to one of 2 groups:(1) sedentary and (2) exercise for 12 weeks. Mice had ad libitum access to food and water. Exercised mice were trained for 4 weeks at 60-70% max speed for 1 hr each session, 5 d/wk. WT sedentary and exercised groups were given antibiotics via oral gavage during the last 7 days of the exercise protocol. At the end of 4 weeks, mice were sacrificed and intestinal tissues were fixed for …