Authors
Noralane M Lindor, Lawrence J Burgart, Olga Leontovich, Richard M Goldberg, Julie M Cunningham, Daniel J Sargent, Catherine Walsh-Vockley, Gloria M Petersen, Michael D Walsh, Barbara A Leggett, Joanne P Young, Melissa A Barker, Jeremy R Jass, John Hopper, Steve Gallinger, Bharati Bapat, Mark Redston, Stephen N Thibodeau
Publication date
2002/2/15
Journal
Journal of clinical oncology
Volume
20
Issue
4
Pages
1043-1048
Publisher
American Society of Clinical Oncology
Description
PURPOSE: To compare microsatellite instability (MSI) testing with immunohistochemical (IHC) detection of hMLH1 and hMSH2 in colorectal cancer.
PATIENTS AND METHODS: Colorectal cancers from 1,144 patients were assessed for DNA mismatch repair deficiency by two methods: MSI testing and IHC detection of hMLH1 and hMSH2 gene products. High-frequency MSI (MSI-H) was defined as more than 30% instability of at least five markers; low-level MSI (MSI-L) was defined as 1% to 29% of loci unstable.
RESULTS: Of 1,144 tumors tested, 818 showed intact expression of hMLH1 and hMSH2. Of these, 680 were microsatellite stable (MSS), 27 were MSI-H, and 111 were MSI-L. In all, 228 tumors showed absence of hMLH1 expression and 98 showed absence of hMSH2 expression: all were MSI-H.
CONCLUSION: IHC in colorectal tumors for protein products hMLH1 and hMSH2 provides a rapid, cost …
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Scholar articles
NM Lindor, LJ Burgart, O Leontovich, RM Goldberg… - Journal of clinical oncology, 2002