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This study aims to evaluate the composition, the antioxidant and antimicrobial activity of the essential oil of Sage officinale, the extraction was carried out by hydro-distillation. The yield of EO is 3.23%. Analysis of Sage officinale essential oil by CPG-MS identified 26 compounds. Among the majority identified compounds of the essential oil of sage are: trans-decalone (20.33%), 1, 8-cineole (15.0%), αthujone (14.89%), viridiflorol (9.91%), trans-β-caryophyllene (9.27%), carvone (6.19%) and β-thujone (5.68%). The study of antioxidant power by the DPPH method showed the existence of an antioxidant activity of the essential oil of dry sage leaves. The evaluation of antibacterial activity revealed the inhibition of bacterial growth for most tested strains that are of different kinds.