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Methods: We used RT-PCR and in-situ hybridization (ISH) to study the expression of AIP orthologues in fruitfly and zebrafish, and we have generated transgenic AIP-knockdown fruitfly strains by RNA interference using GAL4/UAS-system.

Results: FlyBase RNA ISH showed that CG1847 gene is expressed in fruitfly from early stages (stage I). We generated several fruitfly crosses, using two different GAL4 drivers (actin and elav, expressed in all cells or all neurons respectively) and two different CG1847 RNAi lines (same RNAi construct on ChrII and ChrIII respectively). When actin-GAL4 flies were crossed with CG1847-R2 RNAi line no viable adult offspring were observed suggesting that complete AIP-knockdown is lethal. When using the nervous system-specific elav-GAL4driver, we were able to confirm CG1847 knockdown in the viable offsprings. Wing-targeted knockdown of AIP resulted in specific wing …