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Methods

FTC236 cells were treated in vitro with resveratrol (0 to 25 micromolar). Western blot analysis was performed on cell lysates isolated after 2 and 4 days. Cellular growth was measured by MTT assay over 6 days.

Results

Treatment of FTC236 cells with resveratrol caused a notable dose-dependent increase in the Notch1 intracellular domain (NICD), demonstrating successful activation of Notch1 signaling. Resveratrol treatment also significantly reduced FTC cell growth at all time points. The lowest dose of 5 micromolar decreased cellular proliferation 56% after 6 days (p< 0.0001 vs control). This reduction in cellular proliferation was directly proportional to the degree of NICD expression. Western blot analysis also revealed increased expression of cleaved poly (ADP-ribose) polymerase (PARP) and cleaved caspase-3, with concurrent decreased expression of X-linked inhibitor of apoptosis (XIAP), suggesting …