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The three-dimensional (3-D) electron density representation of the ionosphere computed by the assimilative IRI-SIRMUP-P (ISP) model was tested using IONORT (IONOspheric Ray-Tracing), a software tool for calculating a 3-D ray-tracing for high-frequency waves in the ionospheric medium. A radio link was established between Rome (41.8°N, 12.5°E) in Italy, and Chania (35.7°N, 24.0°E) in Greece, within the ISP validity area, and for which oblique soundings are conducted. The ionospheric reference stations, from which the autoscaled foF2 and M(3000)F2 data and real-time vertical electron density profiles were assimilated by the ISP model, were Rome (41.8°N, 12.5°E) and Gibilmanna (37.9°N, 14.0°E) in Italy, and Athens (38.0°N, 23.5°E) in Greece. IONORT was used, in conjunction with the ISP and the International Reference Ionosphere 3-D electron density grids, to synthesize oblique ionograms. The …