Authors
John R Yates, Stephen Speicher, Patrick R Griffin, Tim Hunkapiller
Publication date
1993/11/1
Journal
Analytical biochemistry
Volume
214
Issue
2
Pages
397-408
Publisher
Academic Press
Description
A computer searching algorithm has been used to identify protein sequences in the Protein Information Resource (PIR) database with peptide mass information (mass map) obtained from proteolytic digests of proteins analyzed by microcapillary high-performance liquid chromatography electrospray ionization mass spectrometry. A theoretical analysis of the cytochrome c family demonstrates the ability to identify protein sequences in the PIR database with a high degree of accuracy using a set of six predicted tryptic peptide masses. This method was also applied to experimentally determined peptide masses for a small GTP-binding protein, a protein from pig uterus, the human sex steroid binding protein, and a thermostable DNA polymerase. The results demonstrate that a set of observed masses which is less than 50% of the total number of predicted masses can be used to identify a protein sequence in the …
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Scholar articles
JR Yates, S Speicher, PR Griffin, T Hunkapiller - Analytical biochemistry, 1993