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Estuaries are primary habitat that serves as feeding and nursery grounds for most juvenile marine fish. However, estuaries in Tanzania have been affected by anthropogenic activities in the upstream of the river catchments including the use of these areas as fishing grounds by the artisanal fishers. These human activities contribute to determine the abundance and diversity of OM sources that support the nutrition of fish; and also to define the complexity of the estuarine food webs. The four complementary methods: stomach contents, stable isotopes (Ֆ13C and Ֆ15N) fatty acid (FA) biomarkers and trophic flow modeling approach were used to describe the OM Nutritional sources and trophic interactions among the dominant marine fish in the Pangani estuary. Among the Fish species investigated (Hilsa Kelee, Valamugil buchanani, Arius africanus, Carangoides chrysophrys and Epinephelus malabaricus …