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A thresholding algorithm for nuclear angiocardiographic studies is presented. The algorithm is free of operator intervention. The threshold is defined on the basis of the distribution of picture elements with decreasing count rates during systole. The application is valid for first-pass and equilibrium ECG gated studies. Reproducibility by different operators with various degrees of experience is high; concordance of the results with those of contrast studies is within the published ranges. The method provides a reliable step in full automation and standardization of scintigraphic angiocardiographies.