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A variety of automated classification approaches have been developed to extract species detection information from large bioacoustic datasets. Convolutional neural networks (CNNs) are an image classification technique that can be operated on the spectrogram of an audio recording. Using CNNs for bioacoustic classification negates the need for sophisticated feature extraction techniques; however, CNNs may be sensitive to the parameters used to create spectrograms. We used AlexNet to classify spectrograms of audio clips from 19 species of birdsong. We trained and tested AlexNet with the spectrograms and observed that mean classification accuracy ranged from 88.9% to 96.9% depending on the parameters used to create the spectrogram. Classification accuracy was highest when we used a composite of four spectrograms with different combinations of scales for frequency and amplitude. Classification …