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The purpose of this study was to specify the time when individuals are committed to female to male sex change in the protogynous wrasse, Halichoeres trimaculatus, induced by treatment with the nonsteroidal aromatase inhibitor (AI) Fadrozole. In this study, treatment with AI was carried out by providing adult females with a diet containing 500μg AI/g food for 3 (AI-3), 5 (AI-5), and 10 days (AI-10). We examined the gonadal structure of the fishes histologically at the end of the AI treatment and 30 days after the start of the experiment. At the end of the AI treatment, all individuals in the AI-3 treated group had gonads with degeneration of yolky oocytes, indicating the onset of sex change. Most individuals in the AI-5 treated group had gonads with atretic vitellogenic oocytes, like those in AI-3 treated group, whereas most individuals in the AI-10 treated group had gonads with testicular tissue. At 30 days after the onset of the …