Authors
Christopher Quince, Umer Zeeshan Ijaz, Nick Loman, A Murat Eren, Delphine Saulnier, Julie Russell, Sarah J Haig, Szymon T Calus, Joshua Quick, Andrew Barclay, Martin Bertz, Michael Blaut, Richard Hansen, Paraic McGrogan, Richard K Russell, Christine A Edwards, Konstantinos Gerasimidis
Publication date
2015/12/1
Journal
The American journal of gastroenterology
Volume
110
Issue
12
Pages
1718-1729
Publisher
Nature Publishing Group
Description
OBJECTIVES:
Exploring associations between the gut microbiota and colonic inflammation and assessing sequential changes during exclusive enteral nutrition (EEN) may offer clues into the microbial origins of Crohn’s disease (CD).
METHODS:
Fecal samples (n= 117) were collected from 23 CD and 21 healthy children. From CD children fecal samples were collected before, during EEN, and when patients returned to their habitual diets. Microbiota composition and functional capacity were characterized using sequencing of the 16SrRNAgene and shotgun metagenomics.
RESULTS:
Microbial diversity was lower in CD than controls before EEN (P= 0.006); differences were observed in 36 genera, 141 operational taxonomic units (OTUs), and 44 oligotypes. During EEN, the microbial diversity of CD children further decreased, and the community structure became even more dissimilar than that of controls. Every 10 …
Scholar articles
C Quince, UZ Ijaz, N Loman, MA Eren, D Saulnier… - Official journal of the American College of …, 2015