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DH Wilson, DC Duffy, DM Rissin, CW Kan, TG Campbell, SC Howes, DR Fournier, T Piech, GK Provuncher, PP Patel, AJ Rivnak, EP Ferrell, JD Randall, L Song, DR Walt

2010

Biotechnology

28

595-599

Methodology Introduction Results Conclusion References Page 1 Development of AccuPSATM, a novel digital immunoassay for sub-femtomolar measurement of PSA in post radical prostatectomy patients DH Wilson1, DC Duffy1, DM Rissin1, CW Kan1, TG Campbell1, SC Howes1, DR Fournier1, T. Piech1, GK Provuncher1, PP Patel1, AJ Rivnak1, EP Ferrell1, JD Randall1, L. Song1, DR Walt2. 1Quanterix Corporation, Cambridge, MA, 2Tufts University, Medford, MA. Fourth AACR International Conference on Molecular Diagnostics in Cancer Therapeutic Development, Denver, Colorado, September 27-30, 2010 1. 2. 3. Formation of single immunocomplexes on beads Magnetic beads Capture antibody Detection antibody with biotin tag Antigen Streptavidin-β-galactosidase Thousands of individual beads loaded into wells A B 1. Enzyme substrate 2. Seal silicone gasket 3. Fluorescence imaging . . . . . . . . . . . . . . . . . . . . . . …