Authors
Jeremy Lambert, Ariel Vonk, Muriel Mendes, Linan Song, Danielle Svancara, Shazia Baig, David Rissin, Shuai Nie, David Hanlon, Purvish Patel, David Duffy, Dandan Shan, Lei Chang, Joe Johnson
Publication date
2018/5/1
Journal
The Journal of Immunology
Volume
200
Issue
1_Supplement
Pages
174.16-174.16
Publisher
American Association of Immunologists
Description
Digital ELISA (Enzyme Linked Immunosorbent Assay) based on single molecule arrays (Simoa) has improved sensitivity of traditional ELISA from picomolar (10− 12 M) to femtomolar (10− 15 M), increasing the quality and quantity of biomarkers that can be measured for health and disease. Digital ELISA counts signal generated from single immunocomplexes formed on superparamagnetic beads confined in arrays of femtoliter-sized wells in which fluorescent molecules are highly concentrated.
Scholar articles
J Lambert, A Vonk, M Mendes, L Song, D Svancara… - The Journal of Immunology, 2018