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Endothelium dysfunction has been associated with vascular disorders such as atherosclerosis [1],[2] and thrombosis [3],[4]. The structure and function of vascular endothelial cells are regulated by biophysical and biomechanical interactions on both the apical and basal sides. On the apical surface, hemodynamic cues, such as shear stress, control endothelial function and hemostasis [5]. While on the basement membrane, endothelial cell behavior is governed by the topography of the substrate, cellular morphology and the cytoskeleton organization [6],[7]. Electrical impedance spectroscopy provides a real time evaluation of cell migration, cell-substrate and cell-cell interaction [8]. In the study described within, we introduce a microfluidic platform that induces apical and basal-cell interactions and replicates the length scale and structure of a blood vessel, with the ability to electrically characterize endothelial cell …