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Ultramafic rocks are exposed at the core of a domal structure near a fossil ridge–transform intersection (RTI) in the Troodos ophiolite. A sequence of heavily serpentinized rocks occurs adjacent to a major axis–parallel fault, the Amiandos Fault (AF), which juxtaposes them against gabbro. Previously, serpentinization and faulting were not considered to be related to the Cretaceous ocean spreading history of the Troodos ophiolite, but instead were interpreted as associated with late, emplacement-related tectonics and diapirism. Unusually high δ¹⁸O values previously measured in Troodos serpentine (Srp) supported this view. Here, petrographic and isotope tracers (δD, δ¹⁸O) of water–rock interaction are examined in three profiles across the AF to determine the spatial distribution, temperature, and the type of water involved in serpentinization in the Troodos RTI. Complete serpentinization, widespread chrysotile …