Authors
Laurent Dortet, Remy A Bonnin, Ivana Pennisi, Lauraine Gauthier, Agnes B Jousset, Laura Dabos, R Christopher D Furniss, Despoina AI Mavridou, Pierre Bogaerts, Youri Glupczynski, Anais Potron, Patrick Plesiat, Racha Beyrouthy, Frédéric Robin, Richard Bonnet, Thierry Naas, Alain Filloux, Gerald Larrouy-Maumus
Publication date
2018/12/1
Journal
Journal of Antimicrobial Chemotherapy
Volume
73
Issue
12
Pages
3359-3367
Publisher
Oxford University Press
Description
Background
Polymyxins are currently considered a last-resort treatment for infections caused by MDR Gram-negative bacteria. Recently, the emergence of carbapenemase-producing Enterobacteriaceae has accelerated the use of polymyxins in the clinic, resulting in an increase in polymyxin-resistant bacteria. Polymyxin resistance arises through modification of lipid A, such as the addition of phosphoethanolamine (pETN). The underlying mechanisms involve numerous chromosome-encoded genes or, more worryingly, a plasmid-encoded pETN transferase named MCR. Currently, detection of polymyxin resistance is difficult and time consuming.
Objectives
To develop a rapid diagnostic test that can identify polymyxin resistance and at the same time differentiate between chromosome- and plasmid-encoded resistances.
Methods
We developed a MALDI …
Scholar articles
L Dortet, RA Bonnin, I Pennisi, L Gauthier, AB Jousset… - Journal of Antimicrobial Chemotherapy, 2018