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In response to signals associated with infection or tissue damage, macrophages undergo a series of dynamic phenotypic changes. Here we show that during the response to lipopolysaccharide and interferon-γ stimulation, metabolic reprogramming in macrophages is also highly dynamic. Specifically, the tricarboxylic acid cycle undergoes a two-stage remodelling: the early stage is characterized by a transient accumulation of intermediates including succinate and itaconate, whereas the late stage is marked by the subsidence of these metabolites. The metabolic transition into the late stage is largely driven by the inhibition of the pyruvate dehydrogenase complex (PDHC) and the oxoglutarate dehydrogenase complex (OGDC), which is controlled by the dynamic changes in the lipoylation state of both PDHC and OGDC E2 subunits and phosphorylation of the PDHC E1 subunit. This dynamic metabolic reprogramming …