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Fascinating properties of ZnO nanostructures have created much interest due to their importance in health care and environmental monitoring. Current worldwide production and their wide range of applications signify ZnO to be a representative of multi-functional oxide material. Recent nanotechnological developments have stimulated the production of various forms of ZnO nanostructures such as nano-layers, nanoparticles, nanowires, etc. Due to their enhanced sensing properties, improved binding ability with biomolecules as well as biological activities have enabled them as suitable candidates for the fabrication of biosensor devices in the biomedical arena. In this review, the synthesis of ZnO nanostructures, mechanism of their interaction with biomolecules and their applications as sensors in health care area are discussed considering the biosensors for molecules with small molecular weight, infectious …