Authors
Maurice WJ de Ronde, Maayke GM Kok, Perry D Moerland, Jan Van den Bossche, Annette E Neele, Amalia Halliani, Ingeborg Van der Made, Menno PJ de Winther, Joost CM Meijers, Esther E Creemers, Sara-Joan Pinto-Sietsma
Publication date
2017/8/1
Journal
Atherosclerosis
Volume
263
Pages
377-384
Publisher
Elsevier
Description
Background and aims
The risk of developing cardiovascular disease (CVD) is twice as high among smoking individuals compared to non-smokers. Monocytes are involved in smoking-related atherosclerotic plaque formation. In this study, we investigated whether smokers with an increased risk of developing CVD can be identified on the basis of monocyte-derived miRNA expression levels.
Methods
We performed a miRNA microarray experiment on isolated monocytes from smoking, former smoking and non-smoking individuals in a cohort of patients with premature CVD and healthy controls (Cohort I, n = 76).
Results
We found miR-124-3p to be heterogeneously expressed among all smoking individuals, whereas expression was low in non-smokers. Subsequently, RT-qPCR measurements on whole blood showed that among smoking individuals an increase in miR-124-3p is associated with an increased risk for …
Scholar articles
MWJ de Ronde, MGM Kok, PD Moerland… - Atherosclerosis, 2017