Authors
Senka Ljubojevic-Holzer, Anthony W Herren, Natasa Djalinac, Julia Voglhuber, Stefano Morotti, Michael Holzer, Brent M Wood, Mahmoud Abdellatif, Ingrid Matzer, Michael Sacherer, Snjezana Radulovic, Markus Wallner, Milan Ivanov, Stefan Wagner, Samuel Sossalla, Dirk von Lewinski, Burkert Pieske, Joan Heller Brown, Simon Sedej, Julie Bossuyt, Donald M Bers
Publication date
2020/10/9
Journal
Circulation research
Volume
127
Issue
9
Pages
1159-1178
Publisher
Lippincott Williams & Wilkins
Description
Rationale
CaMKII (Ca2+-Calmodulin dependent protein kinase) δC activation is implicated in pathological progression of heart failure (HF) and CaMKIIδC transgenic mice rapidly develop HF and arrhythmias. However, little is known about early spatio-temporal Ca2+ handling and CaMKII activation in hypertrophy and HF.
Objective
To measure time- and location-dependent activation of CaMKIIδC signaling in adult ventricular cardiomyocytes, during transaortic constriction (TAC) and in CaMKIIδC transgenic mice.
Methods and Results
We used human tissue from nonfailing and HF hearts, 4 mouse lines: wild-type, KO (CaMKIIδ-knockout), CaMKIIδC transgenic in wild-type (TG), or KO background, and wild-type mice exposed to TAC. Confocal imaging and biochemistry revealed disproportional CaMKIIδC activation and accumulation in nuclear and perinuclear versus cytosolic regions at 5 days post-TAC. This …
Total citations
Scholar articles
S Ljubojevic-Holzer, AW Herren, N Djalinac… - Circulation research, 2020