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This article investigates the main barriers to the adoption of B2B e-marketplaces by large enterprises and at the same time the expected benefits that push in the opposite direction towards the adoption, through a case study conducted at the Hellenic Aerospace Industry (HAI), one of the largest industrial enterprises of Greece. The evidence collected from this case has been analyzed using various frameworks from the literature and also the innovation diffusion theory of Rogers, and shed light on the specificity of the large enterprises concerning e-marketplaces adoption.