Authors
Peter D Burbelo, Francis X Riedo, Chihiro Morishima, Stephen Rawlings, Davey Smith, Sanchita Das, Jeffrey R Strich, Daniel S Chertow, Richard T Davey Jr, Jeffrey I Cohen
Publication date
2020/6/29
Journal
The Journal of infectious diseases
Volume
222
Issue
2
Pages
206-213
Publisher
Oxford University Press
Description
Background
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), is associated with respiratory-related disease and death. Assays to detect virus-specific antibodies are important to understand the prevalence of infection and the course of the immune response.
Methods
Quantitative measurements of plasma or serum antibodies to the nucleocapsid and spike proteins were analyzed using luciferase immunoprecipitation system assays in 100 cross-sectional or longitudinal samples from patients with SARS-CoV-2 infection. A subset of samples was tested both with and without heat inactivation.
Results
At >14 days after symptom onset, antibodies against SARS-CoV-2 nucleocapsid protein showed 100% sensitivity and 100% specificity, whereas antibodies to spike protein were detected with 91 …
Scholar articles
PD Burbelo, FX Riedo, C Morishima, S Rawlings… - The Journal of infectious diseases, 2020