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Cellulose-acetate electrophoresis (CAE) provided excellent resolution of allozyme genotypes of P. infestans at the 2 loci Glucose-6-phosphate isomerase (Gpi) and Peptidase (Pep). The cellulose-acetate system has many advantages over traditional starch gel analyses. It is much faster, running in only 15-20 min compared with 16-18 h for starch gels, and because of the short run times the gels do not need to be cooled during electrophoresis. Cellulose acetate is purchased as precast plates, which eliminates the time required to pour starch gels. Both Gpi and Pep can be analysed using a single buffer in the CAE system, whereas 4 buffers are required to resolve these enzymes using starch gels. Finally, only very small amounts of tissue (e.g., 3000 sporangia washed from lesions or infected tuber slices) are required for CAE, so it can be useful even when the fungus has not been isolated into axenic culture. These …