Authors
Annelyse Duvoix, Michaël Schnekenburger, Sylvie Delhalle, Romain Blasius, Patricia Borde-Chiché, Franck Morceau, Mario Dicato, Marc Diederich
Publication date
2004/12/28
Journal
Cancer letters
Volume
216
Issue
2
Pages
207-219
Publisher
Elsevier
Description
Glutathione S-transferases (GST) are involved in cellular protection against xenobiotics, oxidative stress as well as in resistance against chemotherapeutic compounds such as doxorubicin. Levels of human placental type GSTP1-1 are known to be increased in many tumors and hematopoietic diseases. In this work, we compare transcriptional mechanisms in cells that express or not GSTP1-1. Transient transfection assays are used to show that different GST-promoter reporter constructs generate cell-type specific levels of luciferase activity. In expressing cells, transcriptional activity is strongly dependent on AP-1 binding elements within the −65 to −75bp region of the GSTP1 gene as shown by site-directed mutagenesis. Electrophoretic mobility shift assays show that DNA binding activity is exclusively observed in GSTP1-1-expressing cells and is increased after stimulation with hydrogen peroxide, TPA, tert …
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Scholar articles
A Duvoix, M Schnekenburger, S Delhalle, R Blasius… - Cancer letters, 2004