Authors
Tarsis F Gesteira, Vivien Jane Coulson-Thomas, Fernando T Ogata, Eduardo HC Farias, Renan P Cavalheiro, Marcelo A de Lima, Gabriel LA Cunha, Ernesto S Nakayasu, Igor C Almeida, Leny Toma, Helena B Nader
Publication date
2011/12/1
Journal
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics
Volume
1814
Issue
12
Pages
1862-1869
Publisher
Elsevier
Description
Proteoglycans encompass a heterogeneous group of glycoconjugates where proteins are substituted with linear, highly negatively charged glycosaminoglycan chains. Sulphated glycosaminoglycans are ubiquitous to the animal kingdom of the Eukarya domain. Information on the distribution and characterisation of proteoglycans in invertebrate tissues is limited and restricted to a few species. By the use of multidimensional protein identification technology and immunohistochemistry, this study shows for the first time the presence and tissue localisation of different proteoglycans, such as perlecan, aggrecan, and heparan sulphate proteoglycan, amongst others, in organs of the gastropoda Achatina fulica. Through a proteomic analysis of Golgi proteins and immunohistochemistry of tissue sections, we detected the machinery involved in glycosaminoglycan biosynthesis, related to polymer formation (polymerases), as …
Scholar articles
A novel approach for the characterisation of proteoglycans and biosynthetic enzymes in a snail model
TF Gesteira, VJ Coulson-Thomas, FT Ogata… - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2011