Authors
Marco AS Campos, Igor C Almeida, Osamu Takeuchi, Shizuo Akira, Eneida P Valente, Daniela O Procópio, Luiz R Travassos, Jason A Smith, Douglas T Golenbock, Ricardo T Gazzinelli
Publication date
2001/7/1
Journal
The Journal of Immunology
Volume
167
Issue
1
Pages
416-423
Publisher
American Association of Immunologists
Description
Glycosylphosphatidylinositol (GPI) anchors and glycoinositolphospholipids (GIPLs) from parasitic protozoa have been shown to exert a wide variety of effects on cells of the host innate immune system. However, the receptor (s) that are triggered by these protozoan glycolipids has not been identified. Here we present evidence that Trypanosoma cruzi-derived GPI anchors and GIPLs trigger CD25 expression on Chinese hamster ovary-K1 cells transfected with CD14 and Toll-like receptor-2 (TLR-2), but not wild-type (TLR-2-deficient) Chinese hamster ovary cells. The protozoan-derived GPI anchors and GIPLs containing alkylacylglycerol and saturated fatty acid chains or ceramide were found to be active in a concentration range of 100 nM to 1 μM. More importantly, the GPI anchors purified from T. cruzi trypomastigotes, which contain a longer glycan core and unsaturated fatty acids in the sn-2 position of the …
Total citations
Scholar articles
Activation of Toll-like receptor-2 by glycosylphosphatidylinositol anchors from a protozoan parasite
MAS Campos, IC Almeida, O Takeuchi, S Akira… - The Journal of Immunology, 2001