Authors
Meta Roestenberg, Jona Walk, Saskia C van der Boor, Marijke CC Langenberg, Marie-Astrid Hoogerwerf, Jacqueline J Janse, Mikhael Manurung, X Zen Yap, Amanda Fabra García, Jan Pieter R Koopman, Pauline Meij, Els Wessels, Karina Teelen, Youri M van Waardenburg, Marga van de Vegte-Bolmer, Geert Jan van Gemert, Leo G Visser, André JAM van der Ven, Quirijn de Mast, KC Natasha, Yonas Abebe, Tooba Murshedkar, Peter F Billingsley, Tom L Richie, B Kim Lee Sim, Chris J Janse, Stephen L Hoffman, Shahid M Khan, Robert W Sauerwein
Publication date
2020/5/20
Journal
Science translational medicine
Volume
12
Issue
544
Pages
eaaz5629
Publisher
American Association for the Advancement of Science
Description
Immunization with attenuated Plasmodium sporozoites can induce protection against malaria infection, as shown by Plasmodium falciparum (Pf) sporozoites attenuated by radiation in multiple clinical trials. As alternative attenuation strategy with a more homogeneous population of Pf sporozoites (PfSPZ), genetically engineered Plasmodium berghei sporozoites (SPZ) lacking the genes b9 and slarp induced sterile protection against malaria in mice. Consequently, PfSPZ-GA1 Vaccine, a Pf identical double knockout (Pf∆b9∆slarp), was generated as a genetically attenuated malaria parasite vaccine and tested for safety, immunogenicity, and preliminary efficacy in malaria-naïve Dutch volunteers. Dose-escalation immunizations up to 9.0 × 105 PfSPZ of PfSPZ-GA1 Vaccine were well tolerated without breakthrough blood-stage infection. Subsequently, groups of volunteers were immunized three times by direct venous …
Scholar articles
M Roestenberg, J Walk, SC van der Boor… - Science translational medicine, 2020