Authors
Chee Sian Gan, Tiannan Guo, Huoming Zhang, Sai Kiang Lim, Siu Kwan Sze
Publication date
2008/11/7
Journal
Journal of proteome research
Volume
7
Issue
11
Pages
4869-4877
Publisher
American Chemical Society
Description
Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) has been introduced recently for phosphopeptide enrichment. Here we compared ERLIC with the well-established SCX-IMAC for identifying phosphopeptides in EGF-treated A431 cells. The ERLIC approach detected a higher number of phosphopeptides (17 311) than SCX-IMAC (4850), but it only detected 926 unique phosphopeptides compared to 1315 in SCX-IMAC. Only 12% unique phosphopeptides were common to both approaches, suggesting that more comprehensive phosphoproteomes could be generated by complementing SCX-IMAC with ERLIC.
Total citations
Scholar articles
CS Gan, T Guo, H Zhang, SK Lim, SK Sze - Journal of proteome research, 2008