Authors
Siu Kwan Sze, Ying Ge, Han Bin Oh, Fred W McLafferty
Description
New AI-ECD methodologies have also been developed. Plasma ECD is created by introducing 0-10 eV electrons into 10-7 torr nitrogen buffer gas. Then ions are activated by collision (9 eV) with the gas followed by capture of a cooled electron. This was found to be the most effective AI-ECD technique for large protein molecular ions. De novo sequence of ubiquitin can be done in two spectra (73/75 and 72/75 cleavages). 183/258 bonds of carbonic anhydrase have been cleaved in a single spectrum. This extensive sequence information enabled unambiguous identification of two sequence errors (Asp Asn–10 and Asn Asp-
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