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As a discipline, molecular biology has never been static: Newly emerging technologies have always fueled fundamental shifts in the paradigms of biological studies. A number of such large-scale functional studies are already in place as a means of studying the budding yeast, Saccharomyces cerevisiae. With its fully sequenced genome 4 and straightforward genetics, Saccharomyces has provided the template for large-scale projects analyzing gene expression and protein function. Functional studies of the Saccharomyces genome have also encompassed efforts to analyze directly all predicted protein products. While these approaches are all positive steps toward developing efficient functional genomic methodologies, each technique in itself is limited. To address these drawbacks, multifunctional transposon-based mutagenesis systems for the large-scale accumulation of expression …