Authors
Eloi Schmauch, Brian Piening, Maedeh Mohebnasab, Bo Xia, Chenchen Zhu, Jeffrey Stern, Weimin Zhang, Alexa K Dowdell, Jacqueline I Kim, David Andrijevic, Karen Khalil, Ian S Jaffe, Bao-Li Loza, Loren Gragert, Brendan R Camellato, Michelli F Oliveira, Darragh P O’Brien, Han M Chen, Elaina Weldon, Hui Gao, Divya Gandla, Andrew Chang, Riyana Bhatt, Sarah Gao, Xiangping Lin, Kriyana P Reddy, Larisa Kagermazova, Alawi H Habara, Sophie Widawsky, Feng-Xia Liang, Joseph Sall, Alexandre Loupy, Adriana Heguy, Sarah EB Taylor, Yinan Zhu, Basil Michael, Lihua Jiang, Ruiqi Jian, Anita S Chong, Robert L Fairchild, Suvi Linna-Kuosmanen, Minna U Kaikkonen, Vasishta Tatapudi, Marc Lorber, David Ayares, Massimo Mangiola, Navneet Narula, Nader Moazami, Harvey Pass, Ramin S Herati, Adam Griesemer, Manolis Kellis, Michael P Snyder, Robert A Montgomery, Jef D Boeke, Brendan J Keating
Publication date
2024/5/17
Journal
Nature Medicine
Pages
1-13
Publisher
Nature Publishing Group US
Description
In a previous study, heart xenografts from 10-gene-edited pigs transplanted into two human decedents did not show evidence of acute-onset cellular- or antibody-mediated rejection. Here, to better understand the detailed molecular landscape following xenotransplantation, we carried out bulk and single-cell transcriptomics, lipidomics, proteomics and metabolomics on blood samples obtained from the transplanted decedents every 6 h, as well as histological and transcriptomic tissue profiling. We observed substantial early immune responses in peripheral blood mononuclear cells and xenograft tissue obtained from decedent 1 (male), associated with downstream T cell and natural killer cell activity. Longitudinal analyses indicated the presence of ischemia reperfusion injury, exacerbated by inadequate immunosuppression of T cells, consistent with previous findings of perioperative cardiac xenograft dysfunction in …
Scholar articles
E Schmauch, B Piening, M Mohebnasab, B Xia, C Zhu… - Nature Medicine, 2024