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In this paper, uncertainties in the retrieval of satellite surface chlorophyll concentrations in the Mediterranean Sea have been evaluated using both regional and global ocean colour algorithms. The rationale for this effort was to define the most suitable ocean colour algorithm for the reprocessing of the entire SeaWiFS archive over the Mediterranean region where standard algorithms were demonstrated to be inappropriate. Using a large dataset of coincident in situ chlorophyll and optical measurements, covering most of the trophic regimes of the basin, we validated two existing regional algorithms [Bricaud, A., E. Bosc, and D. Antoine, 2002. Algal biomass and sea surface temperature in the Mediterranean Basin — Intercomparison of data from various satellite sensors, and implications for primary production estimates. Remote Sensing of Environment, 81(2–3), 163–178.; D'Ortenzio, F., S. Marullo, M. Ragni, M. R. d …