Authors
Wenjia Xu, Damaris Grain, Sophie Bobet, José Le Gourrierec, Johanne Thévenin, Zsolt Kelemen, Loïc Lepiniec, Christian Dubos
Publication date
2014/4
Journal
New phytologist
Volume
202
Issue
1
Pages
132-144
Description
- In Arabidopsis thaliana, proanthocyanidins (PAs) accumulate in the innermost cell layer of the seed coat (i.e. endothelium, chalaza and micropyle). The expression of the biosynthetic genes involved relies on the transcriptional activity of R2R3‐MYB and basic helix‐loop‐helix (bHLH) proteins which form ternary complexes (‘MBW’) with TRANSPARENT TESTA GLABRA1 (TTG1) (WD repeat protein). The identification of the direct targets and the determination of the nature and spatio‐temporal activity of these MBW complexes are essential steps towards a comprehensive understanding of the transcriptional mechanisms that control flavonoid biosynthesis.
- In this study, various molecular, genetic and biochemical approaches were used.
- Here, we have demonstrated that, of the 12 studied genes of the pathway, only dihydroflavonol‐4‐reductase (DFR), leucoanthocyanidin dioxygenase (LDOX), BANYULS (BAN …
Scholar articles
W Xu, D Grain, S Bobet, J Le Gourrierec, J Thévenin… - New phytologist, 2014