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Materials and Methods:

Twelve diarrheic calves suffering from calf diarrhea in a dairy farm were selected and a total of 36 fecal samples were aseptically collected directly from rectum before, during, and at the end of treatment for each calf to determine the total viable count, total E. coli count and total Salmonella count. A polymerase chain reaction was used for the specific detection of E. coli and Salmonella genus targeting fliC and invA genes, respectively. Antibiotic sensitivity test of the isolated E. coli and Salmonella spp. were performed by the disk diffusion method for eight commonly used antibiotics.

Results:

A total of 36 E. coli (100%) and 12 Salmonella spp.(33%) were isolated from the samples and were confirmed by polymerase chain reaction. Total viable count was found to be ranged from 35× 10 7 to 99× 10 10 colony-forming unit (CFU)/g fecal sample before starting sulfonamide treatment, 34× 10 5 to 25× …