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Background: We hypothesized that the cardioprotective effect of angiotensin II (AngII) type 1 receptor (AT,R) blockade during in vivo ischemia-reperfusion (IR) might be associated with an increase in AngII type 2 receptor (AT₂R) protein, as well as 1,4,5-inositol trisphosphate type 2 receptor (IP₃R) and protein kinase C_ε, (PKC_ε) proteins and cyclic guanosine 3',5' monophosphate (cGMP).

Methods and Results: We studied the effects of the AT₁R blocker, candesartan, on in vivo left ventricular (LV) systolic and diastolic function and remodeling (echocardiogram/Doppler) and hemodynamics during canine reperfused anterior infarction (90-minute ischemia, 120-minute reperfusion), and ex vivo infarct size and AT₁R/AT₂R, IP₃R, and PKC_ε proteins (immunoblots), and cGMP (enzyme immunoassay). Compared with controls, candesartan (1 mg/kg intravenously over 30-minute preischemia) inhibited the AngII pressor …